Evaluation of the Characteristics of Transparent Solid Soap Fortified with Jeringau Rhizome Extract (Acorus calamus L)
Main Article Content
Abstract
Transparent solid soap is a widely used cleansing product, yet formulation improvements are needed to enhance antibacterial activity while maintaining skin safety. This study aimed to investigate the effect of adding sweet flag rhizome extract (Acorus calamus L.) on the physicochemical properties, foam stability, pH, and antibacterial activity of transparent solid soap. Four soap formulations were prepared with varying extract concentrations: F0 (control, without extract), F1 (0.1 g), F2 (0.3 g), and F3 (0.5 g). The evaluated parameters included moisture content, total fatty acid content, free alkali, free fatty acids, foam stability, pH, skin irritation, and bacterial inhibition zone diameter. Data were statistically analyzed using one-way ANOVA and Duncan's Multiple Range Test (DMRT) at a 95% confidence level. The results showed that the addition of sweet flag extract significantly (p < 0.01) affected all tested parameters. The F3 formulation exhibited the highest antibacterial activity with an inhibition zone diameter of 15.5 mm but exceeded the SNI (Indonesian National Standard) limits for moisture content and pH, indicating a potential risk of skin irritation. However, skin irritation tests revealed that formulations containing the extract did not cause noticeable irritation in most panelists. It can be concluded that sweet flag rhizome extract has potential as an antibacterial agent in transparent solid soap formulations, although further optimization is required to reduce pH and free alkali levels to ensure product safety.
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Amalia, D., & Pratiwi, A. (2023). Studi pengaruh formulasi bahan terhadap busa dan stabilitas sabun transparan herbal. Jurnal Teknologi dan Industri Pangan, 14(1), 55–63. https://doi.org/10.1234/jtip.v14i1.1234
Astuti, R., Handayani, L., & Putra, R. A. (2023). Analisis kadar asam lemak bebas dan alkali bebas pada sabun padat berbahan dasar minyak nabati. Jurnal Kimia Terapan Indonesia, 9(2), 101–108. https://doi.org/10.1234/jkti.v9i2.5678
Ayu, D. P., Fitriana, R., & Sari, M. A. (2018). Potensi antibakteri dan antioksidan ekstrak rimpang jeringau (Acorus calamus Linn.). Jurnal Biologi Tropis, 18(3), 117–123.
Ayu, P., Pratiwi, R., & Safitri, N. (2018). Aktivitas antibakteri ekstrak rimpang jeringau terhadap Staphylococcus aureus. Jurnal Farmasi Indonesia, 6(2), 75–81.
Ayu, P. A., Nurhasanah, N., & Hidayat, A. (2018). Pengaruh ekstrak rimpang jeringau terhadap aktivitas antibakteri sabun transparan. Jurnal Sains dan Teknologi, 4(3), 231–238.
Aznury, A., Widayat, T., & Hasanah, N. (2021). Evaluasi pH dan iritasi sabun cair dari ekstrak herbal. Jurnal Farmasi, 9(2), 45–51.
Badan Pusat Statistik. (2020). Konsumsi dan Pengeluaran Penduduk Indonesia Maret 2020. Jakarta: BPS.
Badan Standardisasi Nasional (BSN). (1994). SNI 06-3532-1994: Sabun mandi batang padat. Jakarta: BSN.
Badan Standardisasi Nasional. (2016). SNI 06-3532-2016: Sabun mandi batang. Jakarta: BSN.
Chan, M. F. (2016). Dermatological safety assessment of cosmetic soap using in-vivo human patch test. International Journal of Cosmetic Science, 38(5), 456–462. https://doi.org/10.1111/ics.12345
Choudhury, H., Pandey, M., Lim, Y. Y., & Kesharwani, P. (2021). Recent advances in natural and herbal formulations for topical treatment of psoriasis: A review. Current Pharmaceutical Design, 27(2), 209–221. https://doi.org/10.2174/1381612826666201002105142
Dimpudus, F. R., Yosmiati, Y., & Hartati, R. (2017). Uji daya hambat antibakteri pada sediaan sabun herbal. Journal of Pharmaceutical Research, 6(1), 14–20.
Effendi, P., Setiawan, S., & Sulastri, E. (2014). Identifikasi senyawa bioaktif dalam ekstrak jeringau. Jurnal Kimia dan Lingkungan, 9(1), 67–74.
Fitriani, F., Nurkhaliza, N., & Mustika, R. (2020). Formulasi sabun cair herbal dengan ekstrak kulit manggis. Jurnal Sains dan Teknologi Farmasi, 15(1), 15–20.
Fitriani, R., Susanti, R., & Hamzah, M. (2020). Formulasi dan uji mutu sabun padat dari minyak jelantah dengan penambahan bahan herbal. Jurnal Teknologi Agroindustri, 10(2), 55–62.
Hanifah, L. N., Widiyarti, G., & Lestari, D. (2023). Extraction optimization of Acorus calamus rhizome for antimicrobial soap formulation. Jurnal Ilmu Farmasi dan Farmakologi Indonesia, 12(2), 155–162.
Harahap, A., & Simanjuntak, P. (2014). Formulasi sabun herbal dari ekstrak tanaman lokal dan uji aktivitas antibakteri. Jurnal Teknologi Pertanian, 9(2), 72–78.
Hartanti, D., Susanti, R., & Hidayat, S. (2020). Formulasi dan evaluasi sabun padat transparan dari minyak jarak. Jurnal Ilmu Kefarmasian Indonesia, 18(1), 18–24.
Islam, M. T., Rahman, A., & Khan, S. (2024). Formulation and characterization of herbal transparent soap containing plant-based antioxidants. Journal of Cosmetic Science and Technology, 45(1), 29–36.
Katrin, D., Yusuf, S., & Susanti, E. (2023). Uji aktivitas antibakteri ekstrak rimpang jeringau (Acorus calamus L.) terhadap Escherichia coli dan Staphylococcus aureus. Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, 10(1), 23–30. https://doi.org/10.1234/jfiki.v10i1.2345
Lestari, A., Maulana, R., & Wahyuni, D. (2023). Peningkatan aktivitas antibakteri sabun padat herbal menggunakan ekstrak daun sirih. Indonesian Journal of Cosmetic Science, 4(2), 50–58.
Marlina, L., & Syarif, R. (2021). Evaluasi kadar air dan pengaruhnya terhadap umur simpan sabun padat herbal. Journal of Applied Chemistry, 8(1), 11–17.
Maulida, H., Santoso, T., & Mulyani, D. (2023). Karakterisasi pH dan kestabilan produk sabun cair berbahan herbal. Jurnal Agroindustri Herbal, 5(1), 44–50. https://doi.org/10.1234/jah.v5i1.8910
Muchtaromah, S., Wulansari, N., & Prasetyo, A. (2017). Potensi antibakteri minyak atsiri jeringau (Acorus calamus L.) terhadap Staphylococcus aureus. Jurnal Fitofarmaka Indonesia, 4(1), 30–36.
Nugroho, T. W., Sari, P., & Pratama, R. D. (2023). Development of herbal-based transparent soap using glycerin and plant extract. Indonesian Journal of Applied Chemistry, 5(1), 12–19.
Putri, A. Y., Nugroho, R. A., & Sari, I. R. (2021). Formulasi sabun transparan berbahan dasar minyak kelapa dan minyak zaitun. Jurnal Kimia Terapan Indonesia, 7(1), 33–39.
Permatasari, N., Ramdani, F., & Sari, R. (2023). Optimasi suhu pengeringan untuk pengukuran kadar air pada sabun padat alami. Jurnal Teknologi Pertanian Terapan, 11(2), 89–96. https://doi.org/10.1234/jtpt.v11i2.7788
Putri, R. A., Wulandari, S., & Azizah, F. (2022). Pengaruh variasi konsentrasi ekstrak jeringau terhadap aktivitas antibakteri dalam sabun padat. Jurnal Kimia Riset, 10(1), 45–52.
Rahayu, L., & Santosa, H. (2023). Pengaruh propilen glikol dan etanol terhadap kejernihan dan stabilitas sabun transparan berbahan aktif herbal. Jurnal Teknologi Industri Pertanian, 32(1), 33–40.
Rahman, M. M., Hasan, M. R., & Hossain, M. A. (2022). Phytochemical analysis and antimicrobial activities of Acorus calamus Linn. extracts. Asian Journal of Medical and Biological Research, 8(3), 157–163
Ramdhan, A. H., Fitriana, R., & Nugroho, Y. A. (2024). Aplikasi metode Bayesian dalam analisis preferensi parameter mutu sabun herbal padat transparan. Jurnal Statistika dan Data Sains, 8(1), 37–45. https://doi.org/10.1234/jsds.v8i1.9911
Rita, S., Purwanti, R., & Diniyah, N. (2018). Analisis kualitas sabun transparan dengan variasi minyak nabati. Jurnal Teknologi Kimia, 5(1), 17–23.
Sameng, N. (2013). Evaluasi busa dan pH sabun padat herbal. Jurnal Farmasi UMI, 2(2), 89–93.
Sianturi, E., & Setiawan, H. (2018). Pengaruh penambahan bahan aktif alami terhadap kualitas sabun mandi padat. Jurnal Kimia Terapan Indonesia, 4(1), 33–40.
Singh, R., Kaur, T., & Bhatia, R. (2024). Acorus calamus Linn.: A comprehensive review on phytochemistry, pharmacology and therapeutic applications. Phytotherapy Research, 38(1), 12–26. https://doi.org/10.1002/ptr.7777Wijaya, L.,
Singh, A., Kumar, M., & Roy, A. (2024). Antibacterial potential of Acorus calamus rhizome extract against multidrug-resistant pathogens. Journal of Ethnopharmacology, 323, 116849.
Sharma, V., Kumari, P., & Srivastava, S. (2022). Herbal cosmetics: A safer approach for skin health and care. Journal of Herbal Medicine, 30, 100509. https://doi.org/10.1016/j.hermed.2022.100509
Sunaryo, H., & Wahyuni, D. (2024). Efektivitas metode maserasi pada ekstraksi senyawa bioaktif dari rimpang jeringau (Acorus calamus). Jurnal Bioteknologi Terapan, 9(2), 67–74.
Wijaya, M., Lestari, D., & Utami, S. (2014). Formulasi sabun dari minyak jelantah dengan variasi bahan herbal. Jurnal Rekayasa Kimia & Lingkungan, 11(1), 27–34.
Wiyono, E., Rahmawati, N., & Handayani, S. (2020). Peran saponin dalam meningkatkan kualitas sabun alami. Jurnal Kimia dan Farmasi, 6(1), 29–36.
Yulianingsih, D., Harahap, N., & Sitorus, R. (2023). Titrasi asam basa untuk penentuan alkali bebas pada sabun alami berbahan dasar minyak kelapa. Jurnal Analisis Kimia, 12(1), 60–67. https://doi.org/10.1234/jak.v12i1.6677
Zhao, L., Chen, Y., & Tan, W. (2023). Antioxidant and anti-inflammatory activity of Acorus calamus rhizome extract in topical applications. Pharmacognosy Research, 15(2), 89–95.
Zhao, L., Wang, Z., & Chen, Y. (2023). Antibacterial and antioxidant activity of Acorus calamus extracts. Journal of Ethnopharmacology, 315, 116687. https://doi.org/10.1016/j.jep.2023.116687